| General Information | |
|---|---|
| Description | OneScript® RT Mix for qPCR with gDNAOut is an efficient and fast first-strand cDNA synthesis master mix, suitable for two-step RT-qPCR detection. The 5X OneScript® RT Mix in this product contains all the relevant reagents required for the reverse transcription reaction. It is only needed to add RNA template and DNase/RNase-free water to perform the reverse transcription reaction. The protocol is simple. The OneScript® Reverse Transcriptase in the master mix is a new-generation reverse transcriptase developed based on M-MuLV Reverse Transcriptase, which has reduced RNase H activity and improved thermal stability. The reaction temperature can be settled to up to 55°C, which greatly improves reverse transcription reaction efficiency and tolerance of complex RNA transcripts (high secondary structures or high GC contents), with higher specificity and higher yield. The gDNAOut Mix in this product can completely remove residual genomic DNA in RNA samples and makes the quantitative results more accurate. It is heat sensitive and can be rapidly and irreversibly inactivated by heating at high temperature. So only sample addition is required, and the reaction can be carried out in the same tube to remove genomic DNA contamination and to reverse transcribe the RNA. This cDNA product is specially optimized for qPCR. The primer mix with optimized balance of Random Primers/Oligo d(T)20VN Primers enables that the cDNA synthesis starts at any region of the RNA transcript and with the same reverse transcription efficiency, ensuring high authenticity and reproducibility of qPCR results. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the qPCR reagents can be selected to have high-performance gene expression analysis. |
| Informations d'achat | |
|---|---|
| Certification | RUO |
| French Code (NACRES) | NA.55 |
| UNSPSC code | 41106300 |
| EU customs code | 00000000 |
| Vendor country | FR |
| Human origin | No |
| Marque | |
|---|---|
| Brand | Ozyme |
| Propriétés de produit | |
|---|---|
| MSDS | http://images.bio.ozyme.fr/Web/OZYME/{0db7cafe-ec1e-4f5b-b8c1-11b0304cf4c5}_ozy-onescript-rt-mix-for-qpcrw-gdnaout-msds.pdf |
| Supplier category 3 | RT-qPCR |
| Datasheet/manual | http://images.bio.ozyme.fr/Web/OZYME/{85119243-1ab0-4974-9223-f353597c30c9}_ozy-onescript-wg-DNA-removal-mu.pdf |
| Amplification methods | RT-qPCR |
| Amplification enzymes | Recombinant Taq |
| Usage | |
|---|---|
| Shelf-Life (months) | 12 |
| shipping temperature | -20°C |
| storage temperature | -20°C |
